Anti-IL3RA/CD123 antibody (ab205365)
Key features and details
- Goat polyclonal to IL3RA/CD123
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL3RA/CD123 antibody
See all IL3RA/CD123 primary antibodies -
Description
Goat polyclonal to IL3RA/CD123 -
Host species
Goat -
Specificity
ab205365 is expected to recognize both reported isoforms. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human IL3RA/CD123 aa 100-200 (internal sequence). NCBI Accession No. NP_002174.1 and NP_001254642.1.
Database link: P26951 -
Positive control
- Jurkat and MOLT4 cell lysates.
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General notes
Previously labelled as IL3RA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab205365 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody (ab205365)
Immunohistochemical analysis of paraffin embedded Human colon tissue sections, staining IL3RA / CD123 with ab205365 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot - Anti-IL3RA/CD123 antibody (ab205365)All lanes : Anti-IL3RA/CD123 antibody (ab205365) at 2 µg/ml
Lane 1 : Jurkat cell lysate (in RIPA buffer)
Lane 2 : MOLT4 cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDaPrimary incubation was 1 hour.
