Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107]
See all IL36 gamma/IL-1F9 primary antibodies -
Description
Rabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HaCaT (treated with 20ng/ml IL-1beta and 50ng/ml TNFalpha for 20 hours,) whole cell lysate; His-tagged recombinant human IL36G protein (aa18-169).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23333-107 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution
Lane 1 : HaCaT (human skin keratinocyte), whole cell lysate
Lane 2 : HaCaT treated with 20ng/ml IL-1beta and 50ng/ml TNF-alpha for 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of IL36 gamma/IL-1F9 is induced by IL-1beta and TNF-alpha treatment in HaCaT (PMID: 23095752).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 81 seconds.
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Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution + His-tagged recombinant human IL36G protein (aa18-169), 20 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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