Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23152-241] to IL36 alpha/IL-1F6
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241]
See all IL36 alpha/IL-1F6 primary antibodies
Description

Rabbit monoclonal [EPR23152-241] to IL36 alpha/IL-1F6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Mouse Rat Human
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: RAW 264.7 treated with 1 /ml LPS for 24 hours whole lysate. IHC-P: Mouse esophagus, Human skin and Human esophagus, Rat esophagus tissues. ICC/IF: RAW264.7+LPS cells. Flow Cyt: RAW 264.7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23152-241
Isotype

IgG
Research areas

Western blot - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

All lanes : Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274) at 1/1000 dilution

Lane 1 : Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : RAW 264.7 treated with 1 µg/ml LPS for 24 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 18 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10625660, 28857172)

Exposure time: 3 minutes
Flow Cytometry - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 1ug/ml lipopolysaccharide (LPS) for 24h (Red)/ Untreated control (Green) cells labelling IL36 alpha/IL-1F6 with ab269274 at 1/600 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7+LPS(1ug/ml, 24h)(-/+) cells labelling IL36 alpha/IL-1F6 with ab269274 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing weak cytoplasmic and strong nuclear staining in RAW 264.7 cells treated with lipopolysaccharide (LPS, 1 ug/ml) for 24 hours. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

Immunohistochemical analysis of paraffin-embedded Rat esophagus tissue labeling IL36 alpha/IL-1F6 with ab269274 at 500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat esophagus. The section was incubated with ab269274 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling IL36 alpha/IL-1F6 with ab269274 at 500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human esophagus. The section was incubated with ab269274 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling IL36 alpha/IL-1F6 with ab269274 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skin. The section was incubated with ab269274 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

Immunohistochemical analysis of paraffin-embedded Mouse esophagus tissue labeling IL36 alpha/IL-1F6 with ab269274 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse esophagus (PMID: 20101239). The section was incubated with ab269274 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] (ab269274)

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