Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22198-36] to IL1 Receptor I/IL-1R-1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free
    See all IL1 Receptor I/IL-1R-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22198-36] to IL1 Receptor I/IL-1R-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, EL4.IL2 and bEnd.3 whole cell lysate.

  • General notes

    ab238457 is the carrier-free version of ab229051 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238457 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as IL1 Receptor I.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)
    Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)
    All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051) at 1/1000 dilution

    Lane 1 : EL4.IL-2 (Mouse lymphoma T lymphocyte) cell lysate
    Lane 2 : bEnd.3 (Mouse brain endothelioma ) cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).

     

  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)
    Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)
    All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65,80 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST .

    Exposure times.

    Lane 1: 37 seconds, Lane 2: 3 minutes.

    The 80 kDa band represents the membrane form, while the 65 kDa band is the soluble form (PMID: 17324958, PMID: 28450382).

    Negative control: B16-F10 (PMID: 29445180).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).

     

  • Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)
    Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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