Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 17, 2021

Anti-IL-27-A antibody [EPR18247-101] (ab190831)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18247-101] to IL-27-A
Suitable for: WB, IP
Reacts with: Mouse

Product name

Anti-IL-27-A antibody [EPR18247-101]
See all IL-27-A primary antibodies
Description

Rabbit monoclonal [EPR18247-101] to IL-27-A
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: RAW 264.7 (treated with 100ng/ml LPS for 6h) whole cell lysate. IP: RAW 264.7 (treated with 1µg/ml LPS for 6h) whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18247-101
Isotype

IgG
Research areas

Western blot - Anti-IL-27-A antibody [EPR18247-101] (ab190831)

All lanes : Anti-IL-27-A antibody [EPR18247-101] (ab190831) at 1/1000 dilution

Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (treated with 100ng/ml LPS for 6h) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19620301, PMID: 12121660).

This blot was developed using a higher sensitivity ECL substrate.
Immunoprecipitation - Anti-IL-27-A antibody [EPR18247-101] (ab190831)

IL-27-A was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) (treated with 1μg/ml LPS for 6h) whole cell lysate with ab190831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190831 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: RAW 264.7 (treated with 1μg/ml LPS for 6h ) whole cell lysate 10 μg (Input).
Lane 2: ab190831 IP in RAW 264.7 (treated with 1μg/ml LPS for 6h) whole cell lysate.
Lane 3: : Rabbit monoclonal IgG (ab172730) instead of ab190831 in RAW 264.7 (treated with 1μg/ml LPS for 6h) whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.

The molecular weight observed is consistent with what has been described in the literature (PMID: 19620301, PMID: 12121660).
Anti-IL-27-A antibody [EPR18247-101] (ab190831)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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