Mouse IL-15 ELISA Kit (ab100701)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.33 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-15 ELISA Kit
See all IL-15 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.33 ng/ml - 80 ng/mlRecovery
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 137.2 115% - 150% Plasma 128.5 105% - 148% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s IL-15 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-15 in plasma and cell culture supernatants. (Mouse IL-15 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse IL-15 in normal serum with ab100701, only in serum spiked with Mouse IL-15.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-mouse IL-15 2 vials IL-15 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-15 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
IL-15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL-15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL-15, respectively. Both human and simian IL-15 are active on mouse cells. IL-15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL-15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL-15 and IL-2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL-2 is highly expressed but the expression of IL-15 is not detectable. There is no sequence homology between IL-15 and IL-2, though computer modeling indicates both possess a four alpha helical bundle structure. IL-15 competes for binding sites with IL-2, as both IL2 and IL-15 stimulate the growth of cells through the IL-2 receptor. IL-15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. -
Cellular localization
Isoform IL-15-S48AA: Secreted. Isoform IL-15-S21AA: Cytoplasm. Nucleus. Note: IL-15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. -
Alternative names
- IL 15
- Interleukin 15
- Interleukin15
- MGC9721
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Database links
- Entrez Gene: 16168 Mouse
- SwissProt: P48346 Mouse
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-mouse IL-15 2 vials IL-15 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-15 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
IL-15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL-15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL-15, respectively. Both human and simian IL-15 are active on mouse cells. IL-15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL-15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL-15 and IL-2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL-2 is highly expressed but the expression of IL-15 is not detectable. There is no sequence homology between IL-15 and IL-2, though computer modeling indicates both possess a four alpha helical bundle structure. IL-15 competes for binding sites with IL-2, as both IL2 and IL-15 stimulate the growth of cells through the IL-2 receptor. IL-15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. -
Cellular localization
Isoform IL-15-S48AA: Secreted. Isoform IL-15-S21AA: Cytoplasm. Nucleus. Note: IL-15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. -
Alternative names
- IL 15
- Interleukin 15
- Interleukin15
- MGC9721
-
Database links
- Entrez Gene: 16168 Mouse
- SwissProt: P48346 Mouse
Images
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Typical standard curve
Representative standard curve using ab100701