Anti-IgE antibody [5D4] (ab7295)
![Anti-IgE antibody [5D4] (ab7295)](https://www.abcam.com/ps/products/7/ab7295/Images/ab7295-12740-anti-ige-antibody-5d4-sandwich-elisa.jpg "Anti-IgE antibody [5D4] (ab7295)")
Key features and details
- Mouse monoclonal [5D4] to IgE
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-IgE antibody [5D4]
See all IgE primary antibodies -
Description
Mouse monoclonal [5D4] to IgE -
Host species
Mouse -
Specificity
Specific for IgE Ce2-domain specific -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.
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General notes
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
5D4 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Images
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Sandwich ELISA - Anti-IgE antibody [5D4] (ab7295)Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.
