Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IDH2 (mutated R172W) antibody [WMab-1] (ab264054)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [WMab-1] to IDH2 (mutated R172W)
  • Suitable for: WB, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-IDH2 (mutated R172W) antibody [WMab-1]
    See all IDH2 primary antibodies

  • Description

    Rat monoclonal [WMab-1] to IDH2 (mutated R172W)

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-IDH2 (mutated R172W) antibody [WMab-1] (ab264054)
    Western blot - Anti-IDH2 (mutated R172W) antibody [WMab-1] (ab264054)

    ab264054 used at 1.413ug/ml, followed by Goat Anti-Rat IgG (H+L), HRP) (ab205720) secondary used at a 1/10,000 dilution.


    Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
    Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
    Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
    Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
    Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
    Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
    Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
    Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
    Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
    Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
    Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
    Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
    Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
    Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

    The loading samples are E.coil extracts containing recombinant protein respectively.


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time of 70 seconds.

  • ELISA - Anti-IDH2 (mutated R172W) antibody [WMab-1] (ab264054)
    ELISA - Anti-IDH2 (mutated R172W) antibody [WMab-1] (ab264054)

    ELISA - Anti-IDH2 (mutated R172 W) antibody [WMab-1].

    ab264054 concentration range: 0-5000ng/ml.

    Antigen concentration is 100ng/ml.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used as the secondary at 1/1000 dilution.

  • Anti-IDH2 (mutated R172W) antibody [WMab-1] (ab264054)
    Anti-IDH2 (mutated R172W) antibody [WMab-1] (ab264054)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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