Glyoxalase I Assay Kit (Colorimetric) (ab241019)
Key features and details
- Detection method: Colorimetric
- Sample type: Cell Lysate, Purified protein, Tissue Homogenate
Overview
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Product name
Glyoxalase I Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Homogenate, Purified protein -
Product overview
Glyoxalase I Assay Kit (Colorimetric) (ab241019) utilizes the ability of an active Glyoxalase I (GloI) to catalyze the formation of S-D-lactoylglutathione (SLG) by using two GloI substrates. The formation of the SLG can be tracked using a microplate reader at OD 240 nm.
Our assay kit is simple, sensitive and can detect as low as 0.05 mU of GloI activity in biological samples.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GloI Assay Buffer 1 x 25ml GloI Substrate A 1 x 1.1ml GloI Substrate B (Lyophilized) 1 vial Glyoxalase I (Lyophilized) 1 vial UV transparent plate (96-well) 1 unit -
Research areas
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Function
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. -
Pathway
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2. -
Sequence similarities
Belongs to the glyoxalase I family. - Information by UniProt
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Alternative names
- Aldoketomutase
- glo1
- GLOD1
see all
Images
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Measurement of GloI activity.
Measurement of GloI activity in U937 Cell Lysate (0.6 µg Protein), Hep G2 Cell Lysates (2 µg Protein), Rat Liver tissue extracts (1.5 µg Protein), Rat Kidney tissue extracts (2 µg Protein) & Positive Control.
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Other - Glyoxalase I Assay Kit (Colorimetric) (ab241019)GloI Activity in U937 Cell Lysates, HepG2 Cell Lysates, Rat Liver tissue extracts and Rat Kidney tissue extracts. All assays were performed following kit protocol.