Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)
![Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)](https://www.abcam.com/ps/products/39/ab39713/Images/ab39713-52969-anti-huntingtin-associated-protein-1-antibody-1b6-western-blot.jpg "Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)")
Key features and details
- Mouse monoclonal [1B6] to Huntingtin Associated Protein 1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-Huntingtin Associated Protein 1 antibody [1B6] -
Description
Mouse monoclonal [1B6] to Huntingtin Associated Protein 1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Monkey
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Immunogen
Fusion protein corresponding to Rat Huntingtin Associated Protein 1.
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Positive control
- ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
1B6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)All lanes : Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate - normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
ab39713 detects a band at 85 kDa in mouse and rat brain tissue lysate. This band corresponds to the size of the HAP1-B protein detected in rat brain tissue lysate by Shi-Hua Li et al. (1998). (PMID: 9798945).