Anti-HMGCR antibody [EPR1685(N)] (ab174830)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1685(N)] to HMGCR
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HMGCR antibody [EPR1685(N)]
See all HMGCR primary antibodies -
Description
Rabbit monoclonal [EPR1685(N)] to HMGCR -
Host species
Rabbit -
Specificity
The antibody immunogen shares 93% homology with the mouse protein and 83% homology with the rat protein. We experienced some cross-reactivity variability with mouse and rat samples. -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseRatHuman -
Immunogen
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Positive control
- WB: HepG2, Jurkat, HeLa and TT cell lysates, Mouse and rat brain tissue lysates. MEF whole cell lysate; Mouse liver tissue lysate; Mouse small intestine tissue lysate; Mouse stomach tissue lysate; Mouse hippocampus tissue lysate; Rat liver tissue lysate; Rat stomach tissue lysate. IP: HepG2 whole cell lysate (ab7900).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1685(N) -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Images
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All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 0.4 µg/ml (1:1000 dilution)
Lane 1 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse small intestine tissue lysate
Lane 4 : Mouse stomach tissue lysate
Lane 5 : Mouse hippocampus tissue lysate
Lane 6 : Rat liver tissue lysate
Lane 7 : Rat stomach tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 40 secondsBlocking/Diluting buffer: 5% NFDM/TBST
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All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/3000 dilution (purified)
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : TT (human thyroid carcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/3000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : TT (human thyroid carcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
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ab174830 (purified) at 1/100 immunoprecipitating HMGCR in HepG2 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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