Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13876(B)] to HSDL2 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free
    See all HSDL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13876(B)] to HSDL2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250400 is the carrier-free version of ab181174. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250400 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13876(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    All lanes : Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab181174, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    Immunocytochemistry - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    This data was developed using ab181174, the same antibody clone in a different buffer formulation.Immunofluorescent staining of -20? Acetone fixed HeLa cells labeling HSDL2 using ab181174 at a 1/100 dilution and a Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    This data was developed using ab181174, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HSDL2 using ab181174 at a 1/100 dilution using prediluted HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/10000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 45 kDa



    This data was developed using ab181174, the same antibody clone in a different buffer formulation.

     

  • Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
    Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.