Anti-HS1BP3 antibody - C-terminal (ab200592)
Key features and details
- Rabbit polyclonal to HS1BP3 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HS1BP3 antibody - C-terminal -
Description
Rabbit polyclonal to HS1BP3 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human HS1BP3 aa 300 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_071905.3).
Database link: Q53T59 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab200592 was affinity purified using an epitope specific to HS1BP3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HS1BP3 antibody - C-terminal (ab200592)All lanes : Anti-HS1BP3 antibody - C-terminal (ab200592) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-HS1BP3 antibody - C-terminal (ab200592)Detection of HS1BP3 in immunoprecipitates of HeLa whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab200592 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.