Anti-HOXC8 antibody (ab79690)
Key features and details
- Rabbit polyclonal to HOXC8
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HOXC8 antibody -
Description
Rabbit polyclonal to HOXC8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human HOXC8 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101082) -
Positive control
- This antibody gave a positive signal in Mouse Spinal Cord and Mouse Brain Tissue Lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB MouseAll applications SheepChickenZebrafishApplication Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).Target
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Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
Sequence similarities
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395711 Chicken
- Entrez Gene: 3224 Human
- Entrez Gene: 15426 Mouse
- Omim: 142970 Human
- SwissProt: Q9YH13 Chicken
- SwissProt: P31273 Human
- SwissProt: P09025 Mouse
- Unigene: 664500 Human
see all -
Alternative names
- Homeo box 3A antibody
- Homeo box 8 antibody
- Homeobox 3A antibody
see all
Images
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Western blot - Anti-HOXC8 antibody (ab79690)All lanes : Anti-HOXC8 antibody (ab79690) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-HOXC8 antibody (ab79690)ICC/IF image of ab79690 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79690, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References (0)
ab79690 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HOXC8 antibody (ab79690)All lanes : Anti-HOXC8 antibody (ab79690) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Exposure time: 16 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-HOXC8 antibody (ab79690)ICC/IF image of ab79690 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79690, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
