Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (ab181171)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12171] to HNRPH2/HNRNPH2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-HNRPH2/HNRNPH2 antibody [EPR12171]
See all HNRPH2/HNRNPH2 primary antibodies -
Description
Rabbit monoclonal [EPR12171] to HNRPH2/HNRNPH2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- 293, Jurkat and K562. Human Endometrium tissue. 293 cells.
-
General notes
This product was previously labelled as HNRPH2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12171 -
Isotype
IgG
Images
-
All lanes : Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (ab181171) at 1/10000 dilution
Lane 1 : 293 Cell lysate
Lane 2 : Jurkat Cell lysate
Lane 3 : K562 Cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa
-
Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling HNRPH2/HNRNPH2 with ab181171 at a 1/100 dilution (green). Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Right panel conterstained with Dapi blue.
-
Immunohistochemical analysis of paraffin-embedded Human Endometrium tissue labeling HNRPH2/HNRNPH2 with ab181171at 1/250 dilution. Secondary antibody:anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-