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Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15096] to hnRNP Q - C-terminal
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-hnRNP Q antibody [EPR15096] - C-terminal
    See all hnRNP Q primary antibodies

  • Description

    Rabbit monoclonal [EPR15096] to hnRNP Q - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HCT116, HeLa and Jurkat whole cell lysate (ab7899); Human kidney tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    All lanes : Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946) at 1/50000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HTC116 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

    Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946) at 1/10000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling hnRNP Q with ab184946 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)
    Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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