Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15096] to hnRNP Q - C-terminal
Suitable for: IHC-P, ICC/IF, WB
Reacts with: Human

Product name

Anti-hnRNP Q antibody [EPR15096] - C-terminal
See all hnRNP Q primary antibodies
Description

Rabbit monoclonal [EPR15096] to hnRNP Q - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2, HCT116, HeLa and Jurkat whole cell lysate (ab7899); Human kidney tissue; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15096
Isotype

IgG
Research areas

Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

All lanes : Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946) at 1/50000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HTC116 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946) at 1/10000 dilution + Jurkat cell lysate at 10 µg

Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling hnRNP Q with ab184946 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946)

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