Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140) - BSA and Azide free
  • Suitable for: Dot blot, WB

Overview

  • Product name

    Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free
    See all hnRNP A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Based on sequence homology, we predict that this antibody may react with HNRNPA1L2.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251488 is the carrier-free version of ab208030. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251488 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR20178

  • Isotype

    IgG

  • Research areas

Images

  • Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
    Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
    This data was developed using ab208030, the same antibody clone in a different buffer formulation.Dot blot analysis of hnRNP A1 (citrulline R140) labeled with ab208030 at 1/1000 dilution. Lane 1: hnRNP A1 (citrulline R140) peptide. Lane 2: hnRNP A1 (citrulline R140) noncitrulline peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time : 3 minutes.
  • Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
    Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
    All lanes : Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030) at 1/1000 dilution

    Lane 1 : Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HEK-293T whole cell lysate transfected with GFP tagged PADI2 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
    Lane 3 : Empty vector (vector control) transfected HEK-293T whole cell lysate
    Lane 4 : HEK-293T whole cell lysate transfected with GFP tagged PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab208030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
    Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.