Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
![Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)](https://www.abcam.com/ps/products/251/ab251488/Images/ab251488-2-anti-hnrnp-a1-citrulline-r140-antibody-epr20178-bsa-and-azide-free-dot-blot.jpg "Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140) - BSA and Azide free
- Suitable for: Dot blot, WB
Overview
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Product name
Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free
See all hnRNP A1 primary antibodies -
Description
Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140) - BSA and Azide free -
Host species
Rabbit -
Specificity
Based on sequence homology, we predict that this antibody may react with HNRNPA1L2.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251488 is the carrier-free version of ab208030. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251488 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR20178 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)This data was developed using ab208030, the same antibody clone in a different buffer formulation.Dot blot analysis of hnRNP A1 (citrulline R140) labeled with ab208030 at 1/1000 dilution. Lane 1: hnRNP A1 (citrulline R140) peptide. Lane 2: hnRNP A1 (citrulline R140) noncitrulline peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time : 3 minutes. -
![Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)](https://www.abcam.com/ps/products/251/ab251488/Images/ab251488-399397-anti-hnrnp-a1-citrulline-r140-antibody-epr20178-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)All lanes : Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030) at 1/1000 dilution
Lane 1 : Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T whole cell lysate transfected with GFP tagged PADI2 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
Lane 3 : Empty vector (vector control) transfected HEK-293T whole cell lysate
Lane 4 : HEK-293T whole cell lysate transfected with GFP tagged PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis data was developed using ab208030, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)](https://www.abcam.com/ps/products/251/ab251488/Images/ab251488-3-benefits-of-recombinant-antibodies.png)
Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)