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Epigenetics and Nuclear Signaling Transcription Domain Families HMG Box

Anti-HMGB4 antibody (ab65980)

Anti-HMGB4 antibody (ab65980)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HMGB4
  • Suitable for: WB
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HMGB4 antibody
    See all HMGB4 primary antibodies
  • Description

    Rabbit polyclonal to HMGB4
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human HMGB4 aa 24-41 (N terminal). The human HMGB4 is different to the related mouse and rat sequence by a single amino acid.
    Sequence:

    RNKFKEQQPNTYVGFKEF


    Database link: Q8WW32
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat testicle, brain tissue lysates. Jurkat, HT1080 cell lysates;

Properties

  • Form

    Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-HMGB4 antibody (ab65980)
    Western blot - Anti-HMGB4 antibody (ab65980)
    All lanes : Anti-HMGB4 antibody (ab65980) at 1/2 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat testicular tissue lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : HT1080 whole cell lysate

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Western blot - Anti-HMGB4 antibody (ab65980)
    Western blot - Anti-HMGB4 antibody (ab65980)
    Anti-HMGB4 antibody (ab65980) at 1 µg/ml + rat testicle tissue lysis at 30 µg

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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