Anti-HMGB1 antibody (ab227168)
Key features and details
- Rabbit polyclonal to HMGB1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-HMGB1 antibody
See all HMGB1 primary antibodies -
Description
Rabbit polyclonal to HMGB1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey
-
Immunogen
Recombinant fragment within Human HMGB1 (internal sequence). The exact sequence is proprietary.
Database link: P09429 -
Positive control
- WB: HEK-293T, A431, A-375, HeLa, HepG2, NIH/3T3, JC, BCL-1, C2C12, RAW 264.7 and PC-12 whole cell lysates/extracts. IHC-P: Mouse esophagus and colon tissues; Rat brain stem tissue. ICC/IF: SK-N-SH, HeLa and NIH/3T3 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-HMGB1 antibody (ab227168)All lanes : Anti-HMGB1 antibody (ab227168) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HMGB1 shRNA, whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
-
Western blot - Anti-HMGB1 antibody (ab227168)All lanes : Anti-HMGB1 antibody (ab227168) at 1/3000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
-
Western blot - Anti-HMGB1 antibody (ab227168)Anti-HMGB1 antibody (ab227168) at 1/3000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Predicted band size: 25 kDa12% SDS-PAGE gel.
-
Western blot - Anti-HMGB1 antibody (ab227168)All lanes : Anti-HMGB1 antibody (ab227168) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
-
Western blot - Anti-HMGB1 antibody (ab227168)All lanes : Anti-HMGB1 antibody (ab227168) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody (ab227168)Paraffin-embedded mouse colon tissue stained for HMGB1 using ab227168 at 1/1000 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody (ab227168)Paraffin-embedded rat brain stem tissue stained for HMGB1 using ab227168 at 1/1000 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody (ab227168)Paraffin-embedded mouse esophagus tissue stained for HMGB1 using ab227168 at 1/1000 dilution in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody (ab227168)4% paraformaldehyde-fixed NIH/3T3 (mouse embryonic fibroblast cell line) cells stained for HMGB1 (green) using ab227168 at 1/500 dilution in ICC/IF.
Counterstain: cytoskeleton marker, stained with phalloidin at 1/50 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody (ab227168)4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for HMGB1 (green) using ab227168 at 1/1000 dilution in ICC/IF.
Counterstain: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained with beta Tubulin 3/ Tuj1 antibody at 1/500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody (ab227168)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HMGB1 (green) using ab227168 at 1/1000 dilution in ICC/IF.
Counterstain:cytoskeleton marker, stained with phalloidin at 1/200 dilution. Nuclear Counterstain: Hoechst 33342.
