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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I

Anti-HLAB antibody (ab223657)

Anti-HLAB antibody (ab223657)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to HLAB
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HLAB antibody
    See all HLAB primary antibodies
  • Description

    Goat polyclonal to HLAB
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HLAB aa 100-200 (internal sequence) (Cysteine residue). NP_005505.2.
    Database link: P01889

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Jurkat cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    ab223657 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLAB antibody (ab223657)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLAB antibody (ab223657)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil with ab223657 at 2.5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Western blot - Anti-HLAB antibody (ab223657)
    Western blot - Anti-HLAB antibody (ab223657)
    Anti-HLAB antibody (ab223657) at 0.1 µg/ml + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 40,45 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

    The observed 45kDa band corresponds to the glycosylated form.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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