Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)
![Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.com/ps/products/70/ab70328/Images/ab70328-84890-anti-hla-class-1-abc-antibody-emr8-5-western-blot.jpg "Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)")
Key features and details
- Mouse monoclonal [EMR8-5] to HLA Class 1 ABC
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-HLA Class 1 ABC antibody [EMR8-5]
See all HLA Class 1 ABC primary antibodies -
Description
Mouse monoclonal [EMR8-5] to HLA Class 1 ABC -
Host species
Mouse -
Specificity
ab70328 reacts with the heavy chains of human HLA class 1 A, -B, and -C. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant HLA-A*2402 extracellular domain
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Positive control
- IHC-P: Human tonsil tissue sections
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EMR8-5 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)All lanes : Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)
Lane 1 : A*2402
Lane 2 : A*0101
Lane 3 : A*1101
Lane 4 : A*0201
Lane 5 : A*0207
Lane 6 : A2402
Lane 7 : B*0702
Lane 8 : B*0801
Lane 9 : B*1501
Lane 10 : B*3501
Lane 11 : B*4001
Lane 12 : B*4002
Lane 13 : B*4006
Lane 14 : B*4403
Lane 15 : Cw*0102
Lane 16 : Cw*0801
Lane 17 : Cw*1202
Lane 18 : Cw*1502Western blot analysis of ab70328 reactivity using recominant HLA heavy chains.
Lanes 1-6: HLA-A
Lanes 7-14: HLA-B
Lanes 15-18: HLA-C
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![Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.com/ps/products/70/ab70328/Images/ab70328-84892-anti-hla-class-1-abc-antibody-emr8-5-western-blot.jpg)
Western blot - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)All lanes : Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)
Lane 1 : Human oral cancer cell line OSC20
Lane 2 : HLA-A2402 gene transfected OSC20
Lane 3 : Human Leukemia cell line K562
Lane 4 : Recombinant HLA-A2402 heavy chain
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![Flow Cytometry - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.com/ps/products/70/ab70328/Images/ab70328-84889-anti-hla-class-1-abc-antibody-emr8-5-flow-cytometry.jpg)
Flow Cytometry - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)Overlay histogram showing Jurkat cells stained with ab70328 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70328, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)](https://www.abcam.com/ps/products/70/ab70328/Images/ab70328-448955-antihlaclass1abcantibodyimmunohistochemistrytonsilhuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)IHC image of HLA Class 1 ABC staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab70328, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
