Anti-HIV1 Rev antibody [Rev-6] (ab85529)
![Anti-HIV1 Rev antibody [Rev-6] (ab85529)](https://www.abcam.com/ps/products/85/ab85529/Images/ab85529-100399-anti-hiv1-rev-antibody-rev-6-western-blot.jpg "Anti-HIV1 Rev antibody [Rev-6] (ab85529)")
Key features and details
- Mouse monoclonal [Rev-6] to HIV1 Rev
- Suitable for: WB, ELISA
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 Rev antibody [Rev-6]
See all HIV1 Rev primary antibodies -
Description
Mouse monoclonal [Rev-6] to HIV1 Rev -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Other species -
Immunogen
HIV1 Rev recombinant protein (clade B, HXB-3 isolate) with an N terminal His tag, expressed in bacteria.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 2.94% Sodium citrate, 0.05% Glycerol, 0.00061% Tris -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from supernatants of hybridoma cell cultures by affinity chromatography based on Protein A. -
Clonality
Monoclonal -
Clone number
Rev-6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529) Image from Wong R et al., Retrovirology. 2011 Jun 17;8:47.Fig 8.; doi:10.1186/1742-4690-8-47; 17 June, 2011, Retrovirology, 8:47Representative western blot detecting HIV1 Rev using ab85529 at 1/250 dilution. An HRP-conjugated secondary antibody (1/5000) was used.
HeLa cells stably transduced with an inducible Tet-On HIV-1 system, were untreated or treated for 4-5 h with TG009 or chlorhexidine, followed by induction of expression of the endogenous HIV-1 provirus by addition of doxycycline.
