Anti-HIV1 p17 antibody [2D11] (ab9067)
![Anti-HIV1 p17 antibody [2D11] (ab9067)](https://www.abcam.com/ps/products/9/ab9067/Images/ab9067-16743-anti-hiv1-p17-antibody-2d11-immunofluorescence.jpg "Anti-HIV1 p17 antibody [2D11] (ab9067)")
Key features and details
- Mouse monoclonal [2D11] to HIV1 p17
- Suitable for: WB, ELISA, ICC/IF, Radioimmunoprecipitation
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 p17 antibody [2D11]
See all HIV1 p17 primary antibodies -
Description
Mouse monoclonal [2D11] to HIV1 p17 -
Host species
Mouse -
Specificity
Reacts with Human Immunodeficiency Virus Type 1 (HIV 1) p17 protein -
Tested applications
Suitable for: WB, ELISA, ICC/IF, Radioimmunoprecipitationmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Other Immunogen Type corresponding to HIV1 p17. ab9067 was raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.
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Epitope
Conformational epitope (i.e., pepscan negative) -
General notes
The antibody may be used in indirect immunostaining techniques to detect HIV-1 core protein in fresh or cultured HIV-1 infected cells. Studies on core antigen synthesis and metabolism are performed using Western blotting or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purified from serum-free culture supernatant. -
Primary antibody notes
The antibody may be used in indirect immunostaining techniques to detect HIV-1 core protein in fresh or cultured HIV-1 infected cells. Studies on core antigen synthesis and metabolism are performed using Western blotting or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein. -
Clonality
Monoclonal -
Clone number
2D11 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p17 antibody [2D11] (ab9067) Image from Campbell GR & Spector SA, J Biol Chem. 2011 May 27;286(21):18890-902. Epub 2011 Mar 30. Fig 6.; doi: 10.1074/jbc.M110.206110; May 27, 2011 The Journal of Biological Chemistry, 286, 18890-18902.Immunofluorescence analysis of Macrophages treated with 100 pM 1,25D3 or vehicle control (EtOH) and infected with HIV1Ba-L, staining HIV1 p17 with ab9067.
Cells were fixed and permeabilized in PBS supplemented with paraformaldehyde and 0.1% saponin for 30 min. Samples were incubated with primary antibody and an AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.
