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Immunology Immune System Diseases Antiviral Signaling HIV

Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)

Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11890] to PSIP1/LEDGF - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free
    See all PSIP1/LEDGF primary antibodies
  • Description

    Rabbit monoclonal [EPR11890] to PSIP1/LEDGF - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T and Jurkat cell lysates.
  • General notes

    ab249943 is the carrier-free version of ab177159 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249943 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PSIP1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11890
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Images

  • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 60 kDa



    This data was developed using ab177159, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    Immunocytochemistry - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    This data was developed using ab177159, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of K562 cells labeling PSIP1/LEDGF with ab177159 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    This data was developed using ab177159, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    This data was developed using ab177159, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : PSIP1 knockout HEK293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 60 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab177159).

    Lanes 1-3: Merged signal (red and green). Green - ab177159 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab177159 Anti-PSIP1/LEDGF antibody [EPR11890] was shown to specifically react with PSIP1/LEDGF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266464 (knockout cell lysate ab258144) was used. Wild-type and PSIP1/LEDGF knockout samples were subjected to SDS-PAGE. ab177159 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
    Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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