Anti-Histone H3 antibody - ChIP Grade (ab12079)
Key features and details
- Goat polyclonal to Histone H3 - ChIP Grade
- Suitable for: ChIP, WB, IHC-P
- Reacts with: Mouse, Chicken, Dog, Human, Xenopus laevis, Drosophila melanogaster, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Goat polyclonal to Histone H3 - ChIP Grade -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanIHC-P HumanWB ChickenHuman
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Immunogen
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General notes
This antibody is raised using the same immunogen as our popular anti-Histone H3 rabbit polyclonal - ab1791
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.025% Sodium azide
Constituent: PBS
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
This antibody is raised using the same immunogen as our popular anti-Histone H3 rabbit polyclonal - ab1791 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 antibody - ChIP Grade (ab12079)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab12079 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody - ChIP Grade (ab12079)IHC image of Histone H3 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12079, 5µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Histone H3 antibody - ChIP Grade (ab12079)Anti-Histone H3 antibody - ChIP Grade (ab12079) at 0.5 µg/ml + HeLa Cell Histone prep at 5 µg
Secondary
Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Histone H3 antibody (ab12079) at 0.5 ug/ml, HeLa Cell Histone prep at 5 ug
Secondary
Rabbit polyclonal to Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 15kD -
Western blot - Anti-Histone H3 antibody - ChIP Grade (ab12079)All lanes : Anti-Histone H3 antibody - ChIP Grade (ab12079) at 1 µg/ml
Lane 1 : Chicken S1:29 Cell lysate at 10 µg
Lane 2 : CHO Cell lysate at 10 µg
Lane 3 : Dog MDCK Cell lysate at 10 µg
Lane 4 : Mouse 3T3 Whole Cell Lysate
Lane 5 : Xenopus embryo Cell lysate at 10 µg
Secondary
All lanes : Alexa Fluor 680 Rabbit anti-Goat IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
