Anti-Histone H3 antibody (ab61251)
Key features and details
- Rabbit polyclonal to Histone H3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 -
Host species
Rabbit -
Specificity
We have data to suggest that this antibody can detect unmodified histone H3 and acetylated K9 histone H3. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
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Positive control
- WB: RAW264.7 cell extracts treated with TSA (400nM, hours). IHC-P: Human lung carcinoma.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Histone H3 antibody (ab61251) at 1/500 dilution
Lane 1 : RAW264.7 cell extracts
treated with TSA (400nM, hours)
Lane 2 : RAW264.7 cell extracts
treated with TSA (400nM, hours) with the immunising peptide
Predicted band size: 15 kDa
Observed band size: 15 kDa
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ab61251 at 1/50 - 1/100 dilution staining Histone H3 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.