Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)

Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17613] to Histone H2B (mono methyl K85) - BSA and Azide free
  • Suitable for: PepArr, ICC, WB
  • Reacts with: Mouse, Human

You may also be interested in

Product image
Anti-SGTA antibody (ab96571)
Product image
Anti-CYLD antibody (ab137524)
Product image
Anti-Visfatin antibody - BSA and Azide free (Detector) (ab267450)
Product image
Anti-CD43 antibody [W3/13] (ab22351)

Overview

  • Product name

    Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free
    See all Histone H2B primary antibodies
  • Description

    Rabbit monoclonal [EPR17613] to Histone H2B (mono methyl K85) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: PepArr, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249993 is the carrier-free version of ab177781. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249993 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17613
  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/500 dilution + Mouse ovary tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 5 seconds


    This data was developed using ab177781, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/5000 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 5 seconds


    This data was developed using ab177781, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    All lanes : Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/1000 dilution

    Lane 1 : Human ovary lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : NIH:OVCAR-3 (human ovary adenocarcinoma cell line) cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
    Lane 5 : THP-1 (human monocytic leukemia cell line) cell lysate
    Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 7 : F9 (mouse embryonic testicular cancer cell line) cell lysate
    Lane 8 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 9 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 15 seconds


    This data was developed using ab177781, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

    Under our testing conditions (using whole cell extract and BSA blocking) we detected non-specific bands in addition to the BOI.

  • Peptide Array - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Peptide Array - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    This data was developed using ab177781, the same antibody clone in a different buffer formulation.ab177781 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
  • Immunocytochemistry - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Immunocytochemistry - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    This data was developed using ab177781, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H2B (mono methyl K85) with ab177781 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red). The negative controls are as follows: -ve control 1: ab177781 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution. -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.
  • Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
    Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Properdin/PFC antibody [6E9E6] - BSA and Azide free (ab254276)

  •  
  • Product image

    Mouse PDK4 ELISA Kit (ab215544)

  •  
  • Goat Anti-Pig IgG H&L (TRITC) (ab6912)

  •  
  • Product image

    Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (ab248422)

  •  
  • Product image

    Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (ab268201)

  •  
  • Product image

    Anti-Mammaglobin A antibody [EPR9092] - Low endotoxin, Azide free (ab215384)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.