Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free (ab249993)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17613] to Histone H2B (mono methyl K85) - BSA and Azide free
- Suitable for: PepArr, ICC, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Histone H2B (mono methyl K85) antibody [EPR17613] - BSA and Azide free
See all Histone H2B primary antibodies -
Description
Rabbit monoclonal [EPR17613] to Histone H2B (mono methyl K85) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: PepArr, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249993 is the carrier-free version of ab177781. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249993 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17613 -
Isotype
IgG
Images
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Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/500 dilution + Mouse ovary tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 5 secondsThis data was developed using ab177781, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA/TBST.
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Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/5000 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 5 secondsThis data was developed using ab177781, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA/TBST.
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All lanes : Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/1000 dilution
Lane 1 : Human ovary lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : NIH:OVCAR-3 (human ovary adenocarcinoma cell line) cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
Lane 5 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 7 : F9 (mouse embryonic testicular cancer cell line) cell lysate
Lane 8 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lane 9 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 15 secondsThis data was developed using ab177781, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA/TBST.
Under our testing conditions (using whole cell extract and BSA blocking) we detected non-specific bands in addition to the BOI.
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This data was developed using ab177781, the same antibody clone in a different buffer formulation.ab177781 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
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This data was developed using ab177781, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H2B (mono methyl K85) with ab177781 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red). The negative controls are as follows: -ve control 1: ab177781 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution. -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.
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