Anti-Histone H1.3 antibody [EPR12683] (ab183736)
![Anti-Histone H1.3 antibody [EPR12683] (ab183736)](https://www.abcam.com/ps/products/183/ab183736/Images/ab183736-216198-anti-histone-h13-antibody-epr12683-western-blot.jpg "Anti-Histone H1.3 antibody [EPR12683] (ab183736)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12683] to Histone H1.3
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Histone H1.3 antibody [EPR12683]
See all Histone H1.3 primary antibodies -
Description
Rabbit monoclonal [EPR12683] to Histone H1.3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- T47-D, HeLa & Jurkat whole cell lysate (ab7899), Human cervix carcinoma tissue, HeLa & T47-D cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12683 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H1.3 antibody [EPR12683] (ab183736)All lanes : Anti-Histone H1.3 antibody [EPR12683] (ab183736) at 1/5000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDaBlocking buffer: 5% NFDM/TBST
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![Flow Cytometry - Anti-Histone H1.3 antibody [EPR12683] (ab183736)](https://www.abcam.com/ps/products/183/ab183736/Images/ab183736-216202-anti-histone-h13-antibody-epr12683-flow-cytometry.jpg)
Flow Cytometry - Anti-Histone H1.3 antibody [EPR12683] (ab183736)Flow cytometry analysis of HeLa cells using ab183736 at 1/10 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)](https://www.abcam.com/ps/products/183/ab183736/Images/ab183736-216201-anti-histone-h13-antibody-epr12683-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)](https://www.abcam.com/ps/products/183/ab183736/Images/ab183736-216200-anti-histone-h13-antibody-epr12683-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)Immunofluorescence analysis of HeLa cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] (ab183736)](https://www.abcam.com/ps/products/183/ab183736/Images/ab183736-216199-anti-histone-h13-antibody-epr12683-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] (ab183736)Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-Histone H1.3 antibody [EPR12683] (ab183736)](https://www.abcam.com/ps/products/183/ab183736/Images/ab183736-6-benefits-of-recombinant-antibodies.png)
Anti-Histone H1.3 antibody [EPR12683] (ab183736)
