Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H1.3 antibody [EPR12683] (ab183736)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12683] to Histone H1.3
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H1.3 antibody [EPR12683]
    See all Histone H1.3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12683] to Histone H1.3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • T47-D, HeLa & Jurkat whole cell lysate (ab7899), Human cervix carcinoma tissue, HeLa & T47-D cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12683

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Histone H1.3 antibody [EPR12683] (ab183736)
    Western blot - Anti-Histone H1.3 antibody [EPR12683] (ab183736)
    All lanes : Anti-Histone H1.3 antibody [EPR12683] (ab183736) at 1/5000 dilution

    Lane 1 : T47-D cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa



    Blocking buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-Histone H1.3 antibody [EPR12683] (ab183736)
    Flow Cytometry - Anti-Histone H1.3 antibody [EPR12683] (ab183736)

    Flow cytometry analysis of HeLa cells using ab183736 at 1/10 dilution.  Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution.  Fixative: 2% paraformaldehyde.  Isotype control: Rabbit monoclonal IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)

    Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.  Fixative: 4% paraformaldehyde.  Counter stain: Dapi

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (ab183736)

    Immunofluorescence analysis of HeLa cells labeling Histone H1.3 with ab183736 at 1/500 dilution.  Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] (ab183736)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] (ab183736)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution.  Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Histone H1.3 antibody [EPR12683] (ab183736)
    Anti-Histone H1.3 antibody [EPR12683] (ab183736)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Histone H1.3 antibody [EPR12683] (ab183736)

© 2021 Astana Biomed Group LLP. All rights reserved.