Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha - BSA free
  • Suitable for: IP, ChIP-sequencing, ICC, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free
    See all HIF-2-alpha primary antibodies

  • Description

    Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha - BSA free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ChIP-sequencing, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HIF-2-alpha aa 400-450. The exact sequence is proprietary. NP_001421.2 and Gene ID 2034.
    Database link: Q99814

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272041 is the carrier-free version of ab243861. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Localization of human HIF2-alpha binding sites in immunoprecipitates from subcutaneous tumor 786-O cells by ChIP-Seq using ab243861.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

  • Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells untreated (Left) and treated with CoCl2 (right), labeling HIF-2 alpha (Red) using ab243861 at 1/100 dilution. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/100 dilution. Phalloidin Alexa Fluor® 488conjugated (green) was used as the counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

  • Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-2 alpha using ab243861 at 1/400 dilution in ICC/IF analysis. A HRP-conjugated goat-anti rabiit IgG was used as the secondary. DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-2 alpha using ab243861 at 1/400 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

  • Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)

    HIF-2 alpha was immunoprecipitated from 1 mg HepG2 whole cell lysate with ab243861 at 20 µL per reaction. Western blot was performed on the immunoprecipitate using ab243861 at 1/1000 dilution.

    Lane 1: ab243861 IP in HepG2 cell lysate treated with 200 µM CoCl2.

    Lane 2: ab243861 IP in HepG2 whole cell lysate Mock treated.

    Lane 3: Contol IgG in HepG2 cell lysate treated with 200 µM CoCl2.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

  • Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
    Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)

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