Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HES5 antibody [EPR15578] (ab194111)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15578] to HES5
Suitable for: IHC-P, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-HES5 antibody [EPR15578]
See all HES5 primary antibodies
Description

Rabbit monoclonal [EPR15578] to HES5
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293, C6 and PC-12 cell lysates; Human cerebellum, Human fetal brain, mouse brain, mouse kidney, mouse spleen, rat brain, rat kidney and rat spleen lysates; Human glioblastoma, Human adenocarcinoma of endometrium and mouse cerebral cortex tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15578
Isotype

IgG
Research areas

Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + 293 cell lysate at 10 µg

Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + Human cerebellum lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution + Human fetal brain lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

All lanes : Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lane 7 : C6 cell lysate
Lane 8 : PC-12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-HES5 antibody [EPR15578] (ab194111)

Western blot detection of HES5 in Human cerebellum cell lysate immunoprecipitated using ab194111 at 1/40 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-HES5 antibody [EPR15578] (ab194111)

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