Anti-Hepsin/HPN antibody [EP7654] (ab189246)
![Anti-Hepsin/HPN antibody [EP7654] (ab189246)](https://www.abcam.com/ps/products/189/ab189246/Images/ab189246-366351-anti-hepsin-hpn-antibody-ep7654-western-blot-hepg2-pc-3-hela-human.jpg "Anti-Hepsin/HPN antibody [EP7654] (ab189246)")
Key features and details
- Rabbit monoclonal [EP7654] to Hepsin/HPN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hepsin/HPN antibody [EP7654]
See all Hepsin/HPN primary antibodies -
Description
Rabbit monoclonal [EP7654] to Hepsin/HPN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa, PC-3, Human fetal liver, C6 and Raw 264.7 cell lysates. IHC-P: Human liver and stomach tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP7654 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hepsin/HPN antibody [EP7654] (ab189246)All lanes : Anti-Hepsin/HPN antibody [EP7654] (ab189246) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST
Hepsin is abundantly expressed in HepG2 cells which is consistent with PMID: 1885621 and PMID: 8346233
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![Western blot - Anti-Hepsin/HPN antibody [EP7654] (ab189246)](https://www.abcam.com/ps/products/189/ab189246/Images/ab189246-224588-anti-hepsin-antibody-ep7654-western-blot.jpg)
Western blot - Anti-Hepsin/HPN antibody [EP7654] (ab189246)Anti-Hepsin/HPN antibody [EP7654] (ab189246) at 1/1000 dilution + Human fetal liver at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 45 kDa
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![Western blot - Anti-Hepsin/HPN antibody [EP7654] (ab189246)](https://www.abcam.com/ps/products/189/ab189246/Images/ab189246-224589-anti-hepsin-antibody-ep7654-western-blot.jpg)
Western blot - Anti-Hepsin/HPN antibody [EP7654] (ab189246)All lanes : Anti-Hepsin/HPN antibody [EP7654] (ab189246) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
