Anti-Hepsin/HPN antibody (ab73133)
Key features and details
- Rabbit polyclonal to Hepsin/HPN
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hepsin/HPN antibody
See all Hepsin/HPN primary antibodies -
Description
Rabbit polyclonal to Hepsin/HPN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Human Hepsin/HPN protein (ab114907) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 whole cell lysate.
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General notes
This product was previously labelled as Hepsin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications RabbitHorseCowDogMacaque monkeyOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Plays an essential role in cell growth and maintenance of cell morphology. -
Tissue specificity
Present in most tissues, with the highest level in liver. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 508148 Cow
- Entrez Gene: 3249 Human
- Entrez Gene: 100173788 Orangutan
- Omim: 142440 Human
- SwissProt: P05981 Human
- SwissProt: Q5R5E8 Orangutan
- Unigene: 182385 Human
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Alternative names
- EC 3.4.21. antibody
- HEPS antibody
- HEPS_HUMAN antibody
see all
Images
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Western blot - Anti-Hepsin/HPN antibody (ab73133)Anti-Hepsin/HPN antibody (ab73133) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesHepsin/HPN contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunocytochemistry/ Immunofluorescence - Anti-Hepsin/HPN antibody (ab73133)ab73133 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab73133 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde (4%, 10min) fixed HeLa, Hek293, and HepG2 cell types at 5ug/ml.
Protocols
Datasheets and documents
References (0)
ab73133 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Hepsin/HPN antibody (ab73133)Anti-Hepsin/HPN antibody (ab73133) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesHepsin/HPN contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
-
Immunocytochemistry/ Immunofluorescence - Anti-Hepsin/HPN antibody (ab73133)
ab73133 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab73133 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde (4%, 10min) fixed HeLa, Hek293, and HepG2 cell types at 5ug/ml.
