Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)
![Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)](https://www.abcam.com/ps/products/8/ab8635/Images/ab8635-15077-anti-hepatitis-b-virus-pre-s2-antigen-antibody-s26-western-blot.jpg "Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)")
Key features and details
- Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
- Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB
- Reacts with: Hepatitis B virus
- Isotype: IgG1
Overview
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Product name
Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] -
Description
Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen -
Host species
Mouse -
Specificity
This antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species). -
Tested applications
Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Purified serum HBV surface antigen
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Epitope
aa positions 131-139 (adw nomenclature) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
S26 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) Image courtesy of Alina Macovei by Abreview.All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HEK cells
Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 33,36,39 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes