Anti-Heme Oxygenase 1 antibody (ab137749)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Heme Oxygenase 1 antibody
See all Heme Oxygenase 1 primary antibodies -
Description
Rabbit polyclonal to Heme Oxygenase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-278 of Human Heme Oxygenase 1.
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Positive control
- H1299, HeLa, NIH-3T3, JC, BCL-1, C2C12 and Raw264.7 whole cell lysates, OV90 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/500 dilution
Lane 1 : Wile-type HeLa cell lysate
Lane 2 : Knockout Hela cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
Observed band size: 33 kDa
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Immunohistochemical analysis of human renal carcinaoma using ab137749 at 1/500.
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Immunofluorescent analysis of A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ab137749 diluted at 1:500.
Blue: Hoechst 33342 staining. -
Immunohistochemical analysis of rat spleen using ab137749 at 1/500.
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All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution
Lane 1 : Untreated HeLa whole cell extracts
Lane 2 : Treated HeLa whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
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All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1 : H1299 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded OV90 xenograft, labelling Heme Oxygenase 1 using ab137749 at 1/100 dilution.
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All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lane 4 : C2C12 whole cell lysate
Lane 5 : Raw 264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE