Anti-HEL antibody [5E8] (ab243073)
![Anti-HEL antibody [5E8] (ab243073)](https://www.abcam.com/ps/products/243/ab243073/Images/ab243073-332316-anti-hel-antibody-5e8-western-blot.png "Anti-HEL antibody [5E8] (ab243073)")
Key features and details
- Mouse monoclonal [5E8] to HEL
- Suitable for: WB, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
Overview
-
Product name
Anti-HEL antibody [5E8] -
Description
Mouse monoclonal [5E8] to HEL -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to HEL conjugated to keyhole limpet haemocyanin. Synthetic Hexanoyl modified Keyhole Limpet hemocyanin.
-
Positive control
- WB: HeLa (+/- hydrogen peroxide treatment) cell lysate; Hexanoyl Lysine-BSA. Flow cyt: SH-SY5Y cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
5E8 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-HEL antibody [5E8] (ab243073)All lanes : Anti-HEL antibody [5E8] (ab243073) at 1/1000 dilution
Lane 1 : Hexanoyl Lysine-BSA
Lane 2 : BSA
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
-
![Western blot - Anti-HEL antibody [5E8] (ab243073)](https://www.abcam.com/ps/products/243/ab243073/Images/ab243073-332317-anti-hel-antibody-5e8-western-blot.png)
Western blot - Anti-HEL antibody [5E8] (ab243073)All lanes : Anti-HEL antibody [5E8] (ab243073) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (treated with hydrogen peroxide) cell lysate
Lysates/proteins at 12 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hrs at RT.
Secondary incubation for 1 hr at RT.
Block: 5% Skimmed milk in TBST.
-
![Flow Cytometry - Anti-HEL antibody [5E8] (ab243073)](https://www.abcam.com/ps/products/243/ab243073/Images/ab243073-332318-anti-hel-antibody-5e8-flow-cytometry.png)
Flow Cytometry - Anti-HEL antibody [5E8] (ab243073)Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling HEL with ab243073 at 1/50 dilution for 30 mins on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody (20 mins at RT).