Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HS1Y397-F12] to HCLS1 (phospho Y397)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12]
    See all HCLS1 primary antibodies

  • Description

    Rabbit monoclonal [HS1Y397-F12] to HCLS1 (phospho Y397)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human HCLS1 (phospho Y397). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P14317

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Ramos (treated with thapsigargin) cell extract. Flow cyt: Ramos cells (treated with pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    HS1Y397-F12

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using ab278632 at 0.01µg/mL.
  • Western blot - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Western blot - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    All lanes : Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632) at 0.01 µg/ml

    Lane 1 : Ramos cell extract, untreated
    Lane 2 : Ramos cell extract, treated with 300 nM Thapsigargin for 30 minutes

    Predicted band size: 54 kDa

  • Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Flow cytometric analysis of mouse cells secondary antibody only negative control (blue) or control (red) or stimulated (green) using ab278632 at 0.01µg/mL.
  • Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervandadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278632 at 0.01µg/mL.
  • Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)
    Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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