Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rabbit polyclonal to HADHA
Suitable for: WB, IP
Reacts with: Human
Isotype: IgG

Product name

Anti-HADHA antibody
See all HADHA primary antibodies
Description

Rabbit polyclonal to HADHA
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human HADHA aa 713-763. The exact sequence is proprietary.
Database link: P40939
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate

pH 7 to 8
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab241491 was affinity purified using an epitope specific to HADHA immobilized on solid support.
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-HADHA antibody (ab241491)

All lanes : Anti-HADHA antibody (ab241491) at 0.1 µg/ml

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 15 µg per lane.

Developed using the ECL technique.

Predicted band size: 83 kDa

Exposure time: 10 seconds

Lysates prepared using NETN lysis buffer
Immunoprecipitation - Anti-HADHA antibody (ab241491)

HADHA was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241491 at 6 µg per reaction. Western blot was perfomed on the immunoprecipitate using ab241491 at 0.4 µg/ml.

Lane 1: ab241491 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.

Lysates prepared using NETN lysis buffer.

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