Anti-GSH2 antibody (ab229239)
Key features and details
- Rabbit polyclonal to GSH2
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSH2 antibody -
Description
Rabbit polyclonal to GSH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human GSH2 (N terminal). The exact sequence is proprietary.
Database link: Q9BZM3 -
Positive control
- WB: IMR32 whole cell lysate. IP: IMR32 whole cell lysate. ICC/IF: SK-N-SH cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GSH2 antibody (ab229239)
4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for GSH2 (green) using ab229239 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-GSH2 antibody (ab229239)Anti-GSH2 antibody (ab229239) at 1/500 dilution + IMR32 whole cell lysate/extract at 30 µg
Developed using the ECL technique.
Predicted band size: 32 kDa12% SDS-PAGE
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Immunoprecipitation - Anti-GSH2 antibody (ab229239)GSH2 was immunoprecipitated from IMR32 whole cell lysate with 5μg ab190908. Western blot was performed from the immunoprecipitate using ab229239. Anti-rabbit IgG was used as secondary antibody.
Lane 1: IgG instead of ab229239 in IMR32 whole cell lysate.
Lane 2: ab229239 IP in IMR32 whole cell lysate.