Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
![Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)](https://www.abcam.com/ps/products/251/ab251255/Images/ab251255-457146-anti-grid2-antibody-western-blot-cerebellum-human.jpg "Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15422] to GRID2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GRID2 antibody [EPR15422] - BSA and Azide free
See all GRID2 primary antibodies -
Description
Rabbit monoclonal [EPR15422] to GRID2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251255 is the carrier-free version of ab198499. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251255 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15422 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution + Human cerebellum (boiled) at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 113 kDa
Exposure time: 20 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using ab198499, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)](https://www.abcam.com/ps/products/251/ab251255/Images/ab251255-457145-anti-grid2-antibody-western-blot-brain-rat-mouse.jpg)
Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)All lanes : Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution
Lane 1 : Mouse brain lysate (boiled)
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate (boiled)
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 113 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using ab198499, the same antibody clone in a different buffer formulation.
-
![Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)](https://www.abcam.com/ps/products/251/ab251255/Images/ab251255-399114-anti-grid2-antibody-epr15422-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 3 minutesThis data was developed using ab198499, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)](https://www.abcam.com/ps/products/251/ab251255/Images/ab251255-4-benefits-of-recombinant-antibodies.png)
Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
