Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15422] to GRID2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GRID2 antibody [EPR15422] - BSA and Azide free
    See all GRID2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15422] to GRID2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251255 is the carrier-free version of ab198499. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251255 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
    Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
    Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution + Human cerebellum (boiled) at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 113 kDa


    Exposure time: 20 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using ab198499, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
    Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
    All lanes : Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution

    Lane 1 : Mouse brain lysate (boiled)
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate (boiled)
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 113 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using ab198499, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
    Western blot - Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
    Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa
    Observed band size: 113 kDa


    Exposure time: 3 minutes


    This data was developed using ab198499, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)
    Anti-GRID2 antibody [EPR15422] - BSA and Azide free (ab251255)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.