Anti-GRC5 / PHF2 antibody (ab124434)
Key features and details
- Rabbit polyclonal to GRC5 / PHF2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRC5 / PHF2 antibody
See all GRC5 / PHF2 primary antibodies -
Description
Rabbit polyclonal to GRC5 / PHF2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
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Immunogen
corresponding to GRC5/ PHF2.
(Peptide available asab169566) -
Positive control
- This antibody gave a positive signal in both Human and Mouse Liver tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab124434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 120 kDa). Target
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Function
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. -
Tissue specificity
Widely expressed, including in liver (at protein level). -
Sequence similarities
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger. -
Domain
The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3. -
Post-translational
modificationsPhosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 5253 Human
- Entrez Gene: 18676 Mouse
- Entrez Gene: 306814 Rat
- Omim: 604351 Human
- SwissProt: O75151 Human
- SwissProt: Q9WTU0 Mouse
- Unigene: 211441 Human
- Unigene: 212554 Mouse
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Alternative names
- CENP-35 antibody
- GRC5 antibody
- JHDM1E antibody
see all
Images
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Western blot - Anti-GRC5 / PHF2 antibody (ab124434)All lanes : Anti-GRC5 / PHF2 antibody (ab124434) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The predicted molecular weight of GRC5 / PHF2 is 120 kDa (SwissProt), however we expect to observe a banding pattern around 160 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124434 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab124434 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-GRC5 / PHF2 antibody (ab124434)All lanes : Anti-GRC5 / PHF2 antibody (ab124434) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The predicted molecular weight of GRC5 / PHF2 is 120 kDa (SwissProt), however we expect to observe a banding pattern around 160 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124434 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
