Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Granulin antibody [EPR18539-59] (ab187070)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18539-59] to Granulin
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Mouse

Product name

Anti-Granulin antibody [EPR18539-59]
See all Granulin primary antibodies
Description

Rabbit monoclonal [EPR18539-59] to Granulin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse spleen tissue lysate; J774A.1 whole cell lysate. IHC-P: Mouse lung tissue. ICC/IF: J774A.1 cells. Flow Cytometry: J774A.1 cells. IP: J774A.1 whole cell lysate.
General notes
ab187070 is also recommended for human and rat species for IHC.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18539-59
Isotype

IgG
Research areas

Western blot - Anti-Granulin antibody [EPR18539-59] (ab187070)

Lane 1 : Anti-Granulin antibody [EPR18539-59] (ab187070) at 1/1000 dilution
Lane 2 : Anti-Granulin antibody [EPR18539-59] (ab187070) at 1/5000 dilution

Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage cell line) whole cell lysate
Lane 2 : Mouse spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 63 kDa
Observed band size: 88 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 3 seconds.

Lane 2: 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 23972823). Granulin (Progranulin, PGRN) is a 593-amino acid, 68.5-kDa cysteine-rich protein that is typically secreted in a highly glycosylated 88-kDa form.
Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR18539-59] (ab187070)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) cells labeling Granulin with ab187070 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on J774A.1 cell line.

The nuclear counter statin is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Negative control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-Granulin antibody [EPR18539-59] (ab187070)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) cell line labeling Granulin with ab187070 at 1/1000 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Granulin antibody [EPR18539-59] (ab187070)

Granulin was immunoprecipitated from 0.35 mg of J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) whole cell lysate with ab187070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187070 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: J774A.1 lysate 10 μg (Input).

Lane 2: ab187070 IP in J774A.1 lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187070 in J744A.1 whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granulin antibody [EPR18539-59] (ab187070)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Granulin with ab187070 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Sporadic cytoplasmic staining in mouse lung (PMID: 23959579) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Granulin antibody [EPR18539-59] (ab187070)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com