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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase

Anti-gp78 antibody (ab101284)

Anti-gp78 antibody (ab101284)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to gp78
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-gp78 antibody
    See all gp78 primary antibodies
  • Description

    Rabbit polyclonal to gp78
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human gp78 aa 500-550.
    Database link: NP_001135.3

  • Positive control

    • HeLa and 293T whole cell lysates
  • General notes

    Previously labelled as AMFR. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-gp78 antibody (ab101284)
    Western blot - Anti-gp78 antibody (ab101284)
    All lanes : Anti-gp78 antibody (ab101284) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 73 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-gp78 antibody (ab101284)
    Immunoprecipitation - Anti-gp78 antibody (ab101284)

    Detection of Human gp78 in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab101284 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent Western blotting.

    Detection by chemiluminescence with an exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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