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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15059] to GNE - BSA and Azide free
  • Suitable for: ICC, WB, IP
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-GNE antibody [EPR15059] - BSA and Azide free
    See all GNE primary antibodies
  • Description

    Rabbit monoclonal [EPR15059] to GNE - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human GNE aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y223

  • General notes

    ab251009 is the carrier-free version of ab189927 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251009 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as GLCNE

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15059
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development

Images

  • Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    All lanes : Anti-GNE antibody [EPR15059] - C-terminal (ab189927) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : Human fetal brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab189927, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    Western blot - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    All lanes : Anti-GNE antibody [EPR15059] - C-terminal (ab189927) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    This data was developed using ab189927, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    Immunocytochemistry - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    This data was developed using ab189927, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling GNE with ab189927 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
  • Immunoprecipitation - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    Immunoprecipitation - Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    This data was developed using ab189927, the same antibody clone in a different buffer formulation.Western blot analysis of GNE in A549 cell lysate immunoprecipitated using ab189927 at 1/50 dilution. Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)
    Anti-GNE antibody [EPR15059] - BSA and Azide free (ab251009)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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