Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11083] to Caveolin-3 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free
    See all Caveolin-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11083] to Caveolin-3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249654 is the carrier-free version of ab171752.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Western blot - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    All lanes : Anti-Caveolin-3 antibody [EPR11083] (ab171752) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Mouse muscle lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 17 kDa



    This data was developed using ab171752, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)

    This data was developed using ab171752, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human skeletal muscle (PMID: 11431690). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    This data was developed using ab171752, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on human cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    This data was developed using ab171752, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling Caveolin 3 using ab171752 at 1/10 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    This data was developed using ab171752, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on rat cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    This data was developed using ab171752, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on mouse skeletal muscle (PMID: 11431690). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    This data was developed using ab171752, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and cytoplasmic staining on mouse cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)
    Anti-Caveolin-3 antibody [EPR11083] - BSA and Azide free (ab249654)

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