Anti-GNAS antibody (ab101736)
Key features and details
- Goat polyclonal to GNAS
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GNAS antibody
See all GNAS primary antibodies -
Description
Goat polyclonal to GNAS -
Host species
Goat -
Specificity
ab101736 is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human G protein alpha S aa 78-91 (internal sequence).
Sequence:C-QAARSNSDGEKATK
Database link: NP_000507.1 -
Positive control
- WB: Jurkat cell lysate and mouse and rat brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab101736 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GNAS antibody (ab101736)Anti-GNAS antibody (ab101736) at 1 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. -
Western blot - Anti-GNAS antibody (ab101736)All lanes : Anti-GNAS antibody (ab101736) at 0.1 µg/ml
Lane 1 : Mouse brain tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
