Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GNAS antibody [2A2B7] (ab204996)

Key features and details

  • Mouse monoclonal [2A2B7] to GNAS
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-GNAS antibody [2A2B7]
    See all GNAS primary antibodies

  • Description

    Mouse monoclonal [2A2B7] to GNAS

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human GNAS aa 1-200. Expressed in E.coli.
    Database link: P63092

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human G protein alpha (aa42-188) recombinant protein, G protein alpha (aa42-188)-hIgGFc transfected HEK293 whole cell lysate, A549 cells, Human lung cancer or Human breast cancer tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAS antibody [2A2B7] (ab204996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAS antibody [2A2B7] (ab204996)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) analysis of Human breast cancer tissue labeling G protein alpha with ab204996 at a 1/200 dilution. DAB staining used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAS antibody [2A2B7] (ab204996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAS antibody [2A2B7] (ab204996)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) analysis of Human lung cancer tissue labeling G protein alpha with ab204996 at a 1/200 dilution. DAB staining used.

  • Flow Cytometry - Anti-GNAS antibody [2A2B7] (ab204996)
    Flow Cytometry - Anti-GNAS antibody [2A2B7] (ab204996)

    Flow cytometric analysis of A549 cells labelling Human G protein alpha with ab204996 (green) at a 1/200 dilution and negative control (red).

  • Western blot - Anti-GNAS antibody [2A2B7] (ab204996)
    Western blot - Anti-GNAS antibody [2A2B7] (ab204996)
    All lanes : Anti-GNAS antibody [2A2B7] (ab204996) at 1/500 dilution

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : G protein alpha (aa42-188)-hIgGFc transfected HEK293 whole cell lysate

    Predicted band size: 46 kDa

  • Western blot - Anti-GNAS antibody [2A2B7] (ab204996)
    Western blot - Anti-GNAS antibody [2A2B7] (ab204996)
    Anti-GNAS antibody [2A2B7] (ab204996) at 1/500 dilution + Human G protein alpha (aa42-188) recombinant protein

    Predicted band size: 46 kDa
    Additional bands at: 42.8 kDa. We are unsure as to the identity of these extra bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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