Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8199] to Glycophorin A - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free
    See all Glycophorin A primary antibodies

  • Description

    Rabbit monoclonal [EPR8199] to Glycophorin A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver lysate, Human lung tissue, Human spleen tissue.

  • General notes

    ab226153 is the carrier-free version of ab134111 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab226153 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    Western blot - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    Anti-Glycophorin A antibody [EPR8199] (ab134111) at 1/1000 dilution + Human fetal liver tissue lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 16 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab134111, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    This data was developed using ab134111, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Glycophorin A with ab134111 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    This data was developed using ab134111, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Glycophorin A with ab134111 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    OI-RD Scanning - Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    This data was developed using ab134111, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)
    Anti-Glycophorin A antibody [EPR8199] - BSA and Azide free (ab226153)

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