Anti-Glucokinase antibody (ab137714)
Key features and details
- Rabbit polyclonal to Glucokinase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucokinase antibody
See all Glucokinase primary antibodies -
Description
Rabbit polyclonal to Glucokinase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human Glucokinase aa 1-250.
Database link: P35557 -
Positive control
- WB: HEK-293T, Huh7, Hep3B, A431, H1299, HeLa, HepG2, Molt-4, and Raji whole cell lysate. IHC-P: MCF7 xenograft. ICC/IF: A549 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Glucokinase antibody (ab137714) at 1/1000 dilution
Lane 1 : Huh7 whole cell lysate
Lane 2 : Hep3B whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
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Paraffin-embedded human MCF7 xenograft tissue stained for Glucokinase using ab137714 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Glucokinase antibody (ab137714) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labelling Glucokinase with ab137714 at 1/200 (panel 1) and co-stained with a DNA probe (panel 2).