Anti-Glucocorticoid Receptor antibody (ab227524)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucocorticoid Receptor antibody
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Cow, Cat -
Immunogen
Recombinant fragment within Human Glucocorticoid Receptor (internal sequence). The exact sequence is proprietary.
Database link: P04150 -
Positive control
- WB: HeLa whole cell extract; Jurkat and Raji whole cell lysates. IP: HeLa whole cell extract. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Glucocorticoid Receptor Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 86 kDa
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All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Glucocorticoid Receptor knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tissue stained for Glucocorticoid Receptor using ab227524 at 1/250 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for Glucocorticoid Receptor (green) using ab227524 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Glucocorticoid Receptor was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 µg of ab227524. Western blot was performed from the immunoprecipitate using ab227524 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input).
Lane 2: 2.5 µg preimmune rabbit IgG instead of ab227524 in HeLa whole cell lysate.
Lane 3: ab227524 IP in HeLa whole cell lysate.