Anti-GIRK2 antibody [EPR23841-83] (ab259909)
![Anti-GIRK2 antibody [EPR23841-83] (ab259909)](https://www.abcam.com/ps/products/259/ab259909/Images/ab259909-459590-anti-girk2-antibody-epr2384183-western-blot-lysate.jpg "Anti-GIRK2 antibody [EPR23841-83] (ab259909)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23841-83] to GIRK2
- Suitable for: WB, IP, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GIRK2 antibody [EPR23841-83]
See all GIRK2 primary antibodies -
Description
Rabbit monoclonal [EPR23841-83] to GIRK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt (Intra) -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse, rat and human brain lysate. IHC-P: Human cerebrum and insulinoma tissue. Mouse and rat cerebellum tissue. IHC-Fr: Mouse and rat cerebral cortex, cerebellum and hippocampus tissue. IP: Mouse brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23841-83 -
Isotype
IgG
Images
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Western blot - Anti-GIRK2 antibody [EPR23841-83] (ab259909)All lanes : Anti-GIRK2 antibody [EPR23841-83] (ab259909) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate at 20 µg
Lane 2 : Mouse liver tissue lysate at 40 µg
Lane 3 : Rat brain tissue lysate at 20 µg
Lane 4 : Rat liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
Negative control: liver (PMID: 8929423).
Exposure time: 5.5 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Cytoplasmic staining on human cerebrum (PMID: 8642402).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on mouse cerebral cortex (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunoprecipitation - (ab259909)GIRK2 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 µg with ab259909 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab259909 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 µg.
Lane 2: ab259909 IP in mouse brain tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259909 in mouse brain tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 23 seconds.
Input sample is non-boiled as boiling may cause protein aggregates.The expression of truncated GIRK2 proteins observed around 40KD is consistent with what has been described in the literature (PMID:28951616).
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![Western blot - Anti-GIRK2 antibody [EPR23841-83] (ab259909)](https://www.abcam.com/ps/products/259/ab259909/Images/ab259909-459591-anti-girk2-antibody-epr2384183-western-blot-lysate1.jpg)
Western blot - Anti-GIRK2 antibody [EPR23841-83] (ab259909)All lanes : Anti-GIRK2 antibody [EPR23841-83] (ab259909) at 1/1000 dilution
Lane 1 : Human brain tissue lysate at 20 µg
Lane 2 : Human liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48,40,35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Molecular weight around 40kDa, cleaved forms. (PMID:28951616).
Samples are non-boiled as boiling may cause protein aggregates.
Negative control: liver (PMID: 8929423).
Exposure time: 5.5 seconds.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded human insulinoma tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Cytoplasmic staining on human insulinoma (PMID: 7592809).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Postive staining predominantly on granular layer of mouse cerebellum (PMID: 8642402, PMID: 9023358 ).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Postive staining predominantly on granular layer of rat cerebellum (PMID: 8642402, PMID: 9023358).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded human liver tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: No staining on human liver (PMID: 7592809).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on mouse cerebellum (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on mouse hippocampus (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Negative control: No staining on mouse liver (PMID: 7592809) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebral cortex tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on rat cerebral cortex (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on rat cerebellum (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on rat hippocampus (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Negative control: No staining on rat liver (PMID: 7592809) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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(ab259909)
